Thanks for the response Andre. You wrote:
Did you shut down or suspend the VM prior to updating VMware Workstation? Please check the NTFS permissions and delete any *.lck files or folders in the VM's directory. If this still doesn't help try to start VMware Workstation "As Administrator" to see whether this is a permission issue (e.g. UAC, ...)
I did not have it (or any VM) open prior to updating, there are no *.lck files/folders in the VM's dir and I am/login as the Administrator. The odd thing here is that the other 2 VMs setup the same have no issues.
How do I check the NTFS permissions?